Coquet Lab

Julian Stark et al., Allergy 2022

We set up a model of airway inflammation to dog allergen extracts from dog dander and epithelium.

Dog allergen extracts induced strong Th2 and Th17 cell responses, that we visualised by conventional techniques including single cell RNA-Sequencing.

A recombinant molecule comprised of the lipocalins Can f 1, f 2, f 4 and f 6 attenuated airway inflammation when administered prophylactically.

Junjie Ma, Chris Tibbitt et al.,

We recently undertook a single cell RNA-Sequencing analysis of CD4 T cells and ILC2 from nasal polyp tissue of patients with chronic rhinosinusitis

1. We resolve several canonical subsets of T helper cells including Th2, Treg, Tfh cells and 3 distinct CD4 CTL clusters.

2. CD4 CTL have the highest levels of clonal expansion and have distinct patterns of granzyme expression.

3. Th2 cells and ILC2 are profiled side-by-side, displaying several similarities and some differences. Within Th2 cells, we identify a CD109+IL-10+ state, likely reflective of Tr1 cells.